appSCRIPT cDNA synthesis kit

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£982.90

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10+ In Stock

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Description

For reproducible and unbiased cDNA synthesis from 5 and 3 ends of mRNAs
Contains an ultra-stable reverse transcriptase which can synthesize cDNAs up to 9kb
High cDNA yields from as little as 5pg RNA - easily work with low copy templates
Broad starting template range - use between 5pg - 5µg
The kit contains a buffer which has pre-optimised levels of oligo dT and random hexamers which results in reduced transcript bias for downstream qPCR
Short protocol (30mins only)

Convenient two tube format:

Tube 1: reverse transcriptase with RNase inhibitor to prevent degradation of RNA by contaminating RNases
Tube 2: buffer concentrate containing oligo dT, random hexamers,MgCl₂, dNTPs, enhancers and stabilisers

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